Evidence from GC-TRFLP that Bacterial Communities in Soil Are Lognormally Distributed
2008

Bacterial Communities in Soil Are Lognormally Distributed

Sample size: 1 publication Evidence: moderate

Author Information

Author(s): Doroghazi James R., Buckley Daniel H.

Primary Institution: Cornell University

Hypothesis

Can the species abundance distribution of soil bacterial communities be accurately characterized using GC-TRFLP?

Conclusion

The study found that the lognormal distribution best fits the species abundance distribution of soil bacterial communities.

Supporting Evidence

  • GC-TRFLP identified 731 OTUs in the soil sample.
  • The lognormal distribution was the only model that could not be rejected for the community data.
  • TRFLP analysis resulted in fewer detectable TRFs compared to GC-TRFLP.

Takeaway

Scientists looked at bacteria in soil and found that most types are rare while a few are very common, which is like having a few popular toys and many that nobody plays with.

Methodology

The study used GC-TRFLP to analyze a soil sample and identify bacterial operational taxonomic units (OTUs).

Potential Biases

PCR bias could affect the relative abundance of gene sequences and the accuracy of community SAD estimation.

Limitations

The study's findings may be influenced by PCR bias and the inability to culture most bacteria.

Participant Demographics

The study focused on a single soil sample from a fallow field in Ithaca, NY.

Statistical Information

P-Value

0.657

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0002910

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