CBP/p300 and SIRT1 Are Involved in Transcriptional Regulation of S-Phase Specific Histone Genes
2011
How Histone Genes Are Regulated During the Cell Cycle
publication
Evidence: moderate
Author Information
Author(s): He Hongpeng, Fa-Xing Sun, Chi Luo, Yan Bryk
Primary Institution: Institute of Molecular and Cell Biology, Singapore
Hypothesis
The study investigates the transcriptional regulation of S-phase specific histone genes and the role of CBP/p300 and SIRT1 in this process.
Conclusion
The study concludes that CBP/p300 and SIRT1 play crucial roles in the transcriptional regulation of histone genes during the cell cycle.
Supporting Evidence
- Histone gene transcription is tightly coupled to DNA replication.
- CBP/p300 and SIRT1 are recruited to histone gene promoters in a cell cycle-dependent manner.
- Histone acetylation levels fluctuate during the cell cycle, impacting gene expression.
Takeaway
This study shows that certain proteins help control when histone genes are turned on and off during the cell cycle, which is important for DNA replication.
Methodology
The researchers used RT-qPCR and ChIP assays to analyze histone gene expression and acetylation levels during the cell cycle.
Digital Object Identifier (DOI)
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