High-throughput cell-based screening reveals a role for ZNF131 as a repressor of ERalpha signaling
2008

ZNF131: A New Repressor of Estrogen Receptor Alpha Signaling

Sample size: 570 publication Evidence: moderate

Author Information

Author(s): Han Xiao, Guo Jinhai, Deng Weiwei, Zhang Chenying, Du Peige, Shi Taiping, Ma Dalong

Primary Institution: Chinese National Human Genome Center

Hypothesis

Can ZNF131 regulate the activity of estrogen receptor alpha (ERα)?

Conclusion

ZNF131 represses ligand-dependent ERα transactivation and regulates the expression of the estrogen-responsive gene pS2.

Supporting Evidence

  • ZNF131 was identified as a repressor of ERα mediated transactivation from a library of 570 human cDNA clones.
  • ZNF131 inhibits ligand-dependent transactivation by ERα in a dose-dependent manner.
  • ZNF131 suppresses the expression of pS2, an ERα target gene.

Takeaway

Scientists found a new protein called ZNF131 that helps turn off a signal that can lead to breast cancer when estrogen is present.

Methodology

A high-throughput, cell-based functional screening platform was constructed to identify genes that regulate ERα activity.

Limitations

The study primarily focused on in vitro assays, which may not fully replicate in vivo conditions.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1471-2164-9-476

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