Drosophila Brakeless Interacts with Atrophin and Is Required for Tailless-Mediated Transcriptional Repression in Early Embryos
Author Information
Author(s): Haecker Achim, Qi Dai, Lilja Tobias, Moussian Bernard, Andrioli Luiz Paulo, Luschnig Stefan, Mannervik Mattias
Primary Institution: Wenner-Gren Institute, Stockholm University, Stockholm, Sweden
Hypothesis
How does the Brakeless protein interact with Atrophin and contribute to transcriptional repression during Drosophila embryonic development?
Conclusion
Brakeless is a novel co-repressor required for Tailless-mediated transcriptional repression in early Drosophila embryos.
Supporting Evidence
- Brakeless is required for the function of the Tailless nuclear receptor.
- Brakeless and Atrophin interact in vitro and genetically.
- Mutations in the brakeless gene lead to expanded expression patterns of segmentation genes in Drosophila embryos.
Takeaway
Brakeless helps control how genes are turned off during the early development of fruit fly embryos, which is important for their proper growth.
Methodology
The study involved genetic screens, chromatin immunoprecipitation, and transcriptional assays to analyze the role of Brakeless in gene regulation.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website