Human bocavirus in children with respiratory infections
Author Information
Author(s): Zaghloul Mona Z
Primary Institution: Ain Shams University Hospitals, Cairo, Egypt
Hypothesis
The study aimed to determine the presence of human bocavirus in nasopharyngeal aspirates of infants and to assess acute infection through IgM antibodies.
Conclusion
HBoV infections can be effectively diagnosed in children using PCR and ELISA methods.
Supporting Evidence
- 22% of nasopharyngeal aspirates from patients tested positive for HBoV by PCR.
- 18% of serum samples from patients were positive for HBoV IgM antibodies by ELISA.
- PCR showed higher sensitivity (100%) compared to ELISA (81.8%).
- All control samples were negative for HBoV.
Takeaway
Doctors can find a virus called human bocavirus in kids who have coughs and colds by testing their nose and blood samples.
Methodology
The study involved collecting nasopharyngeal aspirates and serum samples from 100 children with respiratory infections and 50 healthy controls, followed by PCR and ELISA testing.
Limitations
The study was limited to a specific age group and geographic location, which may affect the generalizability of the results.
Participant Demographics
100 patients aged 1 to 5 years (61 males and 39 females) and 50 healthy controls (32 males and 18 females).
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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