Inhibition of NAMPT Reduces Inflammation in Arthritis
Author Information
Author(s): Busso Nathalie, Karababa Mahir, Nobile Massimo, Rolaz Aline, Van Gool Frédéric, Galli Mara, Leo Oberdan, So Alexander, De Smedt Thibaut
Primary Institution: Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland
Hypothesis
NAMPT inhibition can reduce inflammation in collagen-induced arthritis.
Conclusion
Inhibiting NAMPT with APO866 significantly reduces inflammation and arthritis severity in mice.
Supporting Evidence
- Inhibition of NAMPT reduced arthritis severity comparable to etanercept.
- APO866 treatment decreased pro-inflammatory cytokines in affected joints.
- Histological analysis showed reduced inflammation in treated mice.
- APO866 did not cause weight loss or other signs of toxicity.
- Intracellular NAD levels were significantly decreased in inflammatory cells after treatment.
Takeaway
This study shows that a drug called APO866 can help reduce swelling and pain in arthritis by blocking a protein that makes inflammation worse.
Methodology
Mice with collagen-induced arthritis were treated with APO866, and inflammation was assessed through clinical scoring and cytokine measurement.
Potential Biases
Potential bias in clinical scoring as the observer was not blinded to treatment groups.
Limitations
The study was conducted in mice, and results may not directly translate to humans.
Participant Demographics
Male DBA/1 and female or male C57BL/6 mice aged 8–12 weeks.
Statistical Information
P-Value
p=0.0025
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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