Increased collagen production in fibroblasts cultured from irradiated skin and effect of TGF β1– clinical study
2000
Collagen Production in Skin Fibroblasts After Radiotherapy
Sample size: 5
publication
Evidence: moderate
Author Information
Author(s): Illsley M C, Peacock J H, McAnulty R J, Yarnold J R
Primary Institution: Royal Marsden Hospital
Hypothesis
Irradiation affects collagen production in fibroblasts from skin biopsies of patients undergoing radiotherapy.
Conclusion
Irradiated fibroblasts produced more collagen than unirradiated fibroblasts, but the response to TGF β1 varied.
Supporting Evidence
- Collagen production rates were significantly higher in fibroblasts from irradiated skin compared to unirradiated skin.
- The median collagen production rate in irradiated cells was 61.16 pmoles hydroxyproline/105cells/hour.
- The median collagen production rate in unirradiated cells was 39.78 pmoles hydroxyproline/105cells/hour.
- TGF β1 increased collagen production in unirradiated fibroblasts but not in all irradiated fibroblast lines.
Takeaway
When skin is treated with radiation, the cells that make collagen work harder, but sometimes they don't respond to a growth factor that usually helps them.
Methodology
Fibroblasts from irradiated and unirradiated skin were cultured and collagen production was measured under controlled conditions.
Participant Demographics
Patients undergoing radiotherapy at the Royal Marsden Hospital.
Statistical Information
P-Value
0.016
Statistical Significance
p=0.016
Digital Object Identifier (DOI)
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