PTEN/MMAC1 Expression in Melanoma Resection Specimens
Author Information
Author(s): Deichmann M, Thome M, Benner A, Egner U, Hartschuh W, Näher H
Primary Institution: Department of Dermatology, University Clinics of Heidelberg
Hypothesis
Investigating PTEN/MMAC1 expression at mRNA level in melanoma resection specimens compared to acquired melanocytic nevi.
Conclusion
PTEN/MMAC1 expression is not down-regulated in melanomas compared to acquired melanocytic nevi, and some melanomas exhibit mutations and deletions in the PTEN/MMAC1 gene.
Supporting Evidence
- PTEN/MMAC1 expression was not lost in melanoma tissues compared to nevi.
- Three point mutations and two deletions were found in the PTEN/MMAC1 gene in some melanoma samples.
- Immunohistochemical analysis showed distinct nuclear staining of PTEN/MMAC1 in melanoma cells.
Takeaway
This study looked at a gene called PTEN in melanoma samples and found that it wasn't less active in melanoma than in normal skin, but some melanoma samples had changes in the gene.
Methodology
Semi-quantitative RT-PCR and immunohistochemistry were used to analyze PTEN/MMAC1 expression and mutations in melanoma and nevus samples.
Limitations
The study had a relatively low number of tested tissue samples, limiting the power to detect differences.
Statistical Information
P-Value
0.17
Confidence Interval
95% confidence interval 0.81; 3.23
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website