How Salmonella Injects Proteins into Host Cells
Author Information
Author(s): Winnen Brit, Schlumberger Markus C., Sturm Alexander, Schüpbach Kaspar, Siebenmann Stefan, Jenny Patrick, Hardt Wolf-Dietrich
Primary Institution: Institute of Microbiology, ETH Zürich, Zürich, Switzerland
Hypothesis
Is there a hierarchy in the injection timing of effector proteins by Salmonella during host cell invasion?
Conclusion
Salmonella injects effector proteins in a hierarchical manner, with SipA being injected before SptP to avoid functional interference.
Supporting Evidence
- SipA and SopE are injected coequally right after host cell contact.
- SptP injection occurs significantly later than SipA and SopE.
- Computer simulations confirmed the hierarchical injection mechanism.
- Effector proteins compete for transport via a limited number of secretion systems.
Takeaway
Salmonella has a special way of injecting proteins into cells, doing it in a specific order to make sure they work better together.
Methodology
The study used time lapse microscopy and computer modeling to analyze the injection of effector proteins by Salmonella.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website