Detection of 65 kD heat shock protein in cerebrospinal fluid of tuberculous meningitis patients
Author Information
Author(s): Mudaliar Anju V, Kashyap Rajpal S, Purohit Hemant J, Taori Girdhar M, Daginawala Hatim F
Primary Institution: Central India Institute of Medical Sciences
Hypothesis
The study aims to evaluate the diagnostic significance of 65 kD heat shock protein in cerebrospinal fluid of tuberculous meningitis patients.
Conclusion
The presence of 65 kD hsp antigen in the CSF of confirmed and suspected cases of TBM indicates that this protein could serve as a specific diagnostic marker for TBM.
Supporting Evidence
- The indirect ELISA method yielded 84% sensitivity and 90% specificity for the diagnosis of TBM.
- The mean absorbance value of 65 kD hsp antigen in TBM patients was significantly higher than in non-TBM groups.
- 100% positivity for 65 kD hsp antigen was found in confirmed TBM cases.
Takeaway
Doctors can use a special test to find a protein in the fluid around the brain that helps them know if someone has a serious infection called tuberculous meningitis.
Methodology
The study analyzed 160 CSF samples using an indirect ELISA method with monoclonal antibodies against the 65 kD hsp antigen.
Limitations
The study may have limitations in generalizability due to the specific patient population and the reliance on CSF samples.
Participant Demographics
Patients included confirmed TBM cases, clinically suspected TBM cases, non-TBM infectious meningitis, and non-infectious neurological disorders.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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