Proteomic Analysis of Chicken Embryonic Tissues After IBV Infection
Author Information
Author(s): Cao Zhongzan, Han Zongxi, Shao Yuhao, Geng Heyuan, Kong Xiangang, Liu Shengwang
Primary Institution: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China
Hypothesis
What are the changes in protein expression in chicken embryonic trachea and kidney tissues following IBV infection in ovo?
Conclusion
This study provides the first analysis of proteomic changes in chicken embryonic trachea and kidney tissues during IBV infection, enhancing our understanding of the virus's pathogenesis.
Supporting Evidence
- Seventy-two hours after inoculation, IBV-infected embryos showed signs of infection like dwarfing and stunting.
- Analysis revealed 17 differentially expressed proteins in tracheal tissues and 19 in kidney tissues.
- Some proteins were confirmed at the mRNA level using real-time RT-PCR.
- Western blotting confirmed changes in annexin A5 and HSPB1 during IBV infection.
Takeaway
Researchers looked at how a virus affects baby chickens' lungs and kidneys, finding many proteins that change when the virus is present.
Methodology
The study used two-dimensional gel electrophoresis (2-DE) and MALDI-TOF-TOF mass spectrometry to analyze protein expression changes.
Participant Demographics
Eighteen 13-day-old SPF chicken embryos were used, divided into IBV-infected and mock-infected groups.
Statistical Information
P-Value
<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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