Effects of Sphingosine on Neutrophil Free Radical Production
Author Information
Author(s): A. Mouithys-Mickalad, G. Deby-Dupont, M. Hoebeke, M. Mathy-Hartert, M. Lamy, C. Deby
Primary Institution: Centre for the Biochemistry of Oxygen, University of Liège
Hypothesis
Sphingolipids inhibit the activation of the neutrophil NADPH oxidase by the protein kinase C pathway.
Conclusion
Sphingosine and its analogues significantly inhibit free radical production in stimulated neutrophils.
Supporting Evidence
- Sphingosine at concentrations of 5 to 8 3 10-6 M inhibited free radical production by stimulated neutrophils.
- The complete inhibition of ESR signal and CL of stimulated PMN confirms that sphingosine acts at the level of NADPH-oxidase.
- Sphingosine was found to be a better inhibitor compared to classical inhibitors like superoxide dismutase and catalase.
Takeaway
Sphingosine helps stop certain cells in our body from making harmful substances when they are activated.
Methodology
The study used electron spin resonance (ESR) and chemiluminescence (CL) techniques to measure free radical production in neutrophils stimulated by phorbol myristate acetate (PMA).
Participant Demographics
Normal healthy volunteers provided blood for neutrophil isolation.
Want to read the original?
Access the complete publication on the publisher's website