Divergent Effects of Human Cytomegalovirus and Herpes Simplex Virus-1 on Cellular Metabolism
Author Information
Author(s): Vastag Livia, Koyuncu Emre, Grady Sarah L., Shenk Thomas E., Rabinowitz Joshua D.
Primary Institution: Princeton University
Hypothesis
How do human cytomegalovirus (HCMV) and herpes simplex virus type-1 (HSV-1) affect the metabolism of host cells?
Conclusion
HCMV and HSV-1 induce distinct metabolic changes in host cells, with HCMV enhancing glycolytic flux and HSV-1 redirecting metabolism towards pyrimidine nucleotide synthesis.
Supporting Evidence
- HCMV infection increases glycolytic flux and TCA cycle activity.
- HSV-1 infection redirects metabolism towards pyrimidine biosynthesis.
- Both viruses trigger significant metabolic changes in host cells.
Takeaway
Two viruses, HCMV and HSV-1, change how cells make energy and building blocks, but they do it in very different ways.
Methodology
Mass spectrometry was used to analyze the metabolic changes in fibroblast and epithelial host cells infected with HCMV and HSV-1.
Limitations
The study primarily focused on single virus-host cell pairs and used transformed cell lines, which may not fully represent natural infections.
Statistical Information
P-Value
p=0.02 for glucose uptake, p=0.0006 for lactate excretion in HCMV-infected cells
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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