Activation of Human T-Helper/Inducer Cell, T-Cytotoxic Cell, B-Cell, and Natural Killer (NK)-Cells and induction of Natural Killer Cell Activity against K562 Chronic Myeloid Leukemia Cells with Modified Citrus Pectin
2011

Modified Citrus Pectin Activates Immune Cells Against Leukemia

Sample size: 3 publication Evidence: moderate

Author Information

Author(s): Ramachandran Cheppail, Wilk Barry J, Hotchkiss Arland, Chau Hoa, Eliaz Isaac, Melnick Steven J

Primary Institution: Dharma Biomedical LLC

Hypothesis

Can modified citrus pectin (MCP) activate human blood lymphocyte subsets and enhance their activity against leukemia cells?

Conclusion

Modified citrus pectin has immunostimulatory properties that activate functional NK cells against K562 leukemic cells.

Supporting Evidence

  • MCP activated T-cytotoxic cells and B-cells in a dose-dependent manner.
  • MCP-activated NK-cells demonstrated functionality in inducing cancer cell death.
  • MCP showed significant activation of NK-cells at higher concentrations.

Takeaway

This study shows that a substance called modified citrus pectin can help our immune cells fight leukemia by making them more active.

Methodology

Human blood samples were treated with modified citrus pectin and analyzed for lymphocyte activation using flow cytometry.

Potential Biases

Potential bias due to authors' affiliations with the manufacturer of MCP.

Limitations

The study was conducted in vitro with a small sample size and lacks in vivo data.

Participant Demographics

Three unidentified healthy volunteers provided blood samples.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1472-6882-11-59

Want to read the original?

Access the complete publication on the publisher's website

View Original Publication