Rtt107/Esc4 binds silent chromatin and DNA repair proteins using different BRCT motifs
2006
Rtt107/Esc4 and Its Role in DNA Repair and Chromatin Silencing
publication
Evidence: moderate
Author Information
Author(s): David C Zappulla, Arindel S R Maharaj, Jessica J Connelly, Rebecca A Jockusch, Rolf Sternglanz
Primary Institution: Stony Brook University
Hypothesis
The study aimed to determine the mechanism of Rtt107/Esc4 targeted silencing and its biological functions.
Conclusion
Rtt107/Esc4 binds to the silencing protein Sir3 and the DNA repair protein Slx4 via different BRCT motifs, thus providing a bridge linking silent chromatin to DNA repair enzymes.
Supporting Evidence
- Rtt107/Esc4 was identified for its ability to establish silent chromatin.
- The targeted silencing activity of Rtt107/Esc4 was dependent on SIR genes.
- The C-terminal two BRCT motifs of Esc4 were sufficient for targeted silencing.
- Esc4 binds to Slx4, linking DNA repair to silent chromatin.
Takeaway
Rtt107/Esc4 helps control gene silencing and DNA repair in yeast by connecting different proteins that do these jobs.
Methodology
The study involved screening a plasmid library and performing two-hybrid tests to assess protein interactions.
Digital Object Identifier (DOI)
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