Prion Protein and Iron Uptake
Author Information
Author(s): Ajay Singh, Mohan Maradumane L., Isaac Alfred Orina, Luo Xiu, Petrak Jiri, Vyoral Daniel, Singh Neena
Primary Institution: The Department of Pathology, Case Western Reserve University
Hypothesis
Does prion protein (PrPC) mediate cellular iron uptake and transport?
Conclusion
PrPC increases cellular iron levels and influences the transport of iron to ferritin, while mutant forms of PrP alter this process differently.
Supporting Evidence
- PrPC increases intracellular iron levels in neuroblastoma cells.
- Mutant PrP forms show differential effects on cellular iron levels.
- Stimulation of PrP endocytosis increases ferritin iron content.
- Cross-linking of PrP decreases its endocytosis and has minimal effect on ferritin iron.
Takeaway
The prion protein helps cells take in iron, which is important for brain health, but some mutations can mess this up.
Methodology
The study used human neuroblastoma cells to assess the effects of PrPC and mutant PrP forms on cellular iron levels and transport mechanisms.
Limitations
The study does not fully elucidate the precise biochemical pathways of iron modulation by PrP.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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