Release of Tumor Necrosis Factor Alpha in Guinea-Pigs After Antigen Challenge
Author Information
Author(s): D. E. Kelly, M. Denis, D. F. Biggs
Primary Institution: University of Alberta
Hypothesis
Can TNF-alpha be detected in the bronchial alveolar lavage fluid of passively sensitized guinea-pigs after inhalation of an antigen?
Conclusion
TNF-alpha is released into the bronchial alveolar lavage fluid following anaphylactic challenge of passively sensitized guinea-pigs, but requires the presence of a protease inhibitor for detection.
Supporting Evidence
- TNF-alpha was not detectable in BAL fluids without the presence of aprotinin.
- Significant amounts of TNF-alpha were detected at 60, 90, and 120 minutes post-challenge.
- BAL fluid from animals that inhaled saline showed no detectable TNF-alpha.
Takeaway
When guinea-pigs that are allergic to a substance breathe it in, their bodies release a chemical called TNF-alpha, but we can only see it if we use a special helper in the test.
Methodology
Guinea-pigs were passively sensitized to ovalbumin and then exposed to aerosols of ovalbumin or control substances, followed by bronchial alveolar lavage to measure TNF-alpha levels.
Limitations
The study could not measure IL-1 or IL-6 due to toxicity of the BAL fluids to the assays used.
Participant Demographics
Female Hartley strain guinea-pigs, SPF quality, weight range 320-400 g.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
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