Cis-urocanic acid and its effects on UV-B exposed corneal cells
Author Information
Author(s): Jauhonen Hanna-Mari, Kauppinen Anu, Paimela Tuomas, Laihia Jarmo K., Leino Lasse, Salminen Antero, Kaarniranta Kai
Primary Institution: Kuopio University Hospital
Hypothesis
UV-B radiation causes cell damage through an increase in transcription factor activity and that cis-UCA may protect the exposed corneal epithelial cells through alterations in this activity.
Conclusion
Cis-UCA suppresses the JNK signaling pathway, providing potential for treating UV-B-induced inflammatory defects in human corneal cells.
Supporting Evidence
- Cis-UCA inhibited the binding of c-Jun and c-Fos but not that of NF-κB.
- UV-B increased the levels of phospho-c-Jun and phospho-JNK, which were attenuated by cis-UCA.
- Cis-UCA alleviated UV-B-induced apoptosis and proliferative decline in human corneal cells.
Takeaway
Cis-urocanic acid helps protect eye cells from damage caused by UV light by blocking certain harmful signals.
Methodology
HCE-2 cells were exposed to UV-B and cis-UCA, and various assays were performed to measure DNA binding of transcription factors and cell viability.
Limitations
The study was conducted in vitro, and further in vivo studies are required.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Want to read the original?
Access the complete publication on the publisher's website