Testing 454 Sequencing on Barley Genome
Author Information
Author(s): Wicker Thomas, Schlagenhauf Edith, Graner Andreas, Close Timothy J, Keller Beat, Stein Nils
Primary Institution: Institute of Plant Biology, University of Zürich
Hypothesis
Can 454 sequencing be an efficient and cost-effective alternative to traditional ABI-Sanger sequencing in repetitive genomes?
Conclusion
454 pyrosequencing allows rapid and cost-effective sequencing of gene-containing portions of large and complex genomes.
Supporting Evidence
- 454 sequencing provided a much more even coverage of the BAC clones than ABI-Sanger sequencing.
- All gene-containing regions were covered efficiently and at high quality with 454 sequencing.
- The combination of 454 sequencing with ABI-Sanger sequencing can result in high-quality finished genomic sequences.
Takeaway
This study shows that a new sequencing method can quickly and cheaply read the important parts of big plant genomes, like barley.
Methodology
The study used 454 sequencing technology to sequence four barley BAC clones and compared the results to ABI-Sanger sequencing.
Limitations
The assembly of repetitive sequences was problematic, and finished BAC sequences were not achieved solely with 454 technology.
Digital Object Identifier (DOI)
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