In Situ Enzyme Activity in the Dissolved and Particulate Fraction of the Fluid from Four Pitcher Plant Species of the Genus Nepenthes
2011

Enzyme Activity of Pitcher Plants

Sample size: 86 publication Evidence: high

Author Information

Author(s): Takeuchi Yayoi, Salcher Michaela M., Ushio Masayuki, Shimizu-Inatsugi Rie, Kobayashi Masaki J., Diway Bibian, von Mering Christian, Pernthaler Jakob, Shimizu Kentaro K.

Primary Institution: Institute of Plant Biology, University of Zurich, Zurich, Switzerland

Hypothesis

What is the density of free-living microbes in the pitcher fluid of Nepenthes?

Conclusion

The study found that high enzyme activity in the pitcher fluid of Nepenthes species aids in the rapid decomposition of prey for nutrient use.

Supporting Evidence

  • High bacterial density was observed within the fluids, ranging from 7×10^6 to 2.2×10^8 cells ml−1.
  • All tested enzymes showed activity that exceeded that observed in aquatic environments.
  • Filtration treatment significantly decreased enzyme activity in all samples.

Takeaway

Pitcher plants have special fluids that help them digest insects, and this study shows that the fluids are full of enzymes that break down food quickly.

Methodology

The study measured enzyme activity in pitcher fluid samples from four Nepenthes species, comparing untreated and filtered samples to assess dissolved versus particle-bound enzyme activity.

Limitations

The study did not focus on the effect of pitcher age and prey species on enzyme activity.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0025144

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