Mapping the Genetic Architecture of the Adaptive Integrated Stress Response in S. cerevisiae
2024
Mapping the Genetic Architecture of the Adaptive Integrated Stress Response in Yeast
publication
Author Information
Author(s): Baum Rachel, Kim Jinyoung, Muller Ryan Y, Ingolia Nicholas
Primary Institution: Cold Spring Harbor Laboratory
Hypothesis
How do genetic perturbations change the dynamics of the integrated stress response in budding yeast?
Conclusion
Genetic perturbations can significantly alter the dynamics of the integrated stress response in yeast, affecting cell growth and ISR activation.
Supporting Evidence
- The study profiles changes in ISR dynamics across thousands of genetic perturbations.
- Treatment with sulfometuron methyl was used to measure ISR reporter expression.
- Depletion of aminoacyl-tRNA synthetases reduced cell growth and activated ISR.
- Impaired ribosome biogenesis reduced basal ISR activity.
Takeaway
This study looks at how changing genes in yeast affects how they respond to stress, helping us understand how cells keep themselves healthy.
Methodology
The study used CRISPR interference with barcoded expression reporter sequencing to profile ISR dynamics across thousands of genetic perturbations.
Digital Object Identifier (DOI)
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