Two-Metal-Ion Mechanism in KdsB Enzyme for Lipopolysaccharide Biosynthesis
Author Information
Author(s): Schmidt Helgo, Mesters Jeroen R., Wu Jing, Woodard Ronald W., Hilgenfeld Rolf, Mamat Uwe
Primary Institution: Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Lübeck, Germany
Hypothesis
Does the KdsB enzyme utilize a two-metal-ion mechanism for Kdo activation in lipopolysaccharide biosynthesis?
Conclusion
The study provides evidence that two magnesium ions are present in the active site of the KdsB enzyme, supporting the two-metal-ion hypothesis.
Supporting Evidence
- The study shows that KdsB forms dimers and is active at high temperatures.
- AA-LCKS exhibited maximum activity at 90°C and a pH optimum of 9.0.
- Two magnesium ions were found in the active site, which is crucial for the enzyme's function.
Takeaway
The KdsB enzyme, which helps bacteria survive, uses two magnesium ions to activate a sugar needed for its protective outer layer.
Methodology
The enzyme was kinetically characterized and its crystal structure was determined at a resolution of 2.10 Å.
Digital Object Identifier (DOI)
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