Inactivation of SARS-CoV-2 Pseudovirus with D614G Mutation using Nitric Oxide-Plasma Activated Water
Author Information
Author(s): Patel Paritosh, Kaushik Neha, Acharya Tirtha Raj, Lenka Sudakshya S., Ghosh Soujanya, Wahab Rizwan, Verma Suresh K., Choi Eun Ha, Kaushik Nagendra Kumar
Primary Institution: Kwangwoon University
Hypothesis
Can nitric oxide-plasma activated water effectively inactivate SARS-CoV-2 pseudovirus expressing the D614G spike protein mutation?
Conclusion
Nitric oxide-plasma activated water can effectively inactivate the SARS-CoV-2 pseudovirus with the D614G mutation, reducing its infectivity by 71%.
Supporting Evidence
- NOx-PAW inhibited infection by 71%.
- NOx-PAW was non-toxic to cells at high concentrations.
- Structural changes in the spike protein were induced by NOx-PAW.
- NOx species in NOx-PAW impaired the function of the mutated spike protein.
- High prevalence of the D614G mutation was observed in SARS-CoV-2 variants.
Takeaway
This study shows that a special type of water, treated with nitric oxide, can kill a virus that has changed in a way that makes it more infectious.
Methodology
The study used nitric oxide-plasma activated water to treat SARS-CoV-2 pseudovirus and assessed its infectivity in HEK-293T-hACE2 cells.
Limitations
The study primarily relied on in vitro experiments, which may not fully represent in vivo conditions.
Participant Demographics
HEK-293T cells expressing human angiotensin-converting enzyme 2 (hACE2).
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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