Rapid Sterilization of Blood Products with Radiation
Author Information
Author(s): Melemenidis Stavros, Nguyen Khoa D., Baraceros-Pineda Rosella, Barclay Cherie K., Bautista Joanne, Lau Hubert, Ashraf M. Ramish, Manjappa Rakesh, Dutt Suparna, Soto Luis Armando, Katila Nikita, Lau Brianna, Visvanathan Vignesh, Yu Amy S., Surucu Murat, Skinner Lawrie B., Engleman Edgar G., Loo Billy W., Pham Tho D.
Primary Institution: Cold Spring Harbor Laboratory
Hypothesis
Can ultra-high dose rate radiation effectively sterilize apheresis blood products while maintaining their quality?
Conclusion
The study demonstrates a new rapid blood sterilization technique that maintains platelet counts and antibody binding after irradiation.
Supporting Evidence
- 1 kGy irradiation of bacteria-spiked platelets reduced E.coli growth by 2.7-log without significant change of platelet count.
- 5 kGy or higher produced complete growth suppression.
- The estimated sterilization dose was 2.3 kGy, corresponding to 31% platelet count reduction.
- A 25 kGy virus sterilizing dose to CCP produced a 9.2% average drop of RBD-specific IgG binding.
Takeaway
This study shows that using radiation can clean blood products from germs without hurting them too much, which could help keep people safe.
Methodology
A clinical linear accelerator was used to deliver ultra-high dose rate irradiation to blood components, followed by assays to assess bacterial growth and antibody binding.
Digital Object Identifier (DOI)
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