Using network pharmacology and molecular docking technology, proteomics and experiments were used to verify the effect of Yigu decoction (YGD) on the expression of key genes in osteoporotic mice
2025

Effects of Yigu Decoction on Osteoporosis in Mice

Sample size: 18 publication 10 minutes Evidence: moderate

Author Information

Author(s): K. Yan, Zhang Rui-Kun, Wang Jia-Xin, Chen Hai-Feng, Zhang Yang, Cheng Feng, Jiang Yi, Wang Min, Wu Ziqi, Chen Xiao-Gang, Chen Zhi-Neng, Li Gui-Jin, Yao Xin-Miao

Primary Institution: The Third Affiliated Hospital of Zhejiang Chinese Medical University

Hypothesis

Yigu decoction (YGD) can prevent and treat osteoporosis by affecting key gene expressions in osteoporotic mice.

Conclusion

YGD may promote osteoblast proliferation and protect osteoblast function through the HIF-1α/VEGF/GLUT1 signaling pathway.

Supporting Evidence

  • YGD contains active components like stigmasterol and flavonoids that are effective against osteoporosis.
  • Animal experiments showed significant improvement in bone mineral density after YGD treatment.
  • Proteomics analysis indicated that YGD affects multiple signaling pathways related to bone metabolism.

Takeaway

Yigu decoction helps mice with weak bones get stronger by making their bone-building cells work better.

Methodology

The study used network pharmacology, molecular docking, proteomics, and animal experiments to analyze the effects of YGD on osteoporosis.

Limitations

The study only partially verified the molecular docking and binding of all active components to related proteins.

Participant Demographics

18 female C57BL/6 mice, 5 weeks old.

Statistical Information

P-Value

p<0.01

Statistical Significance

p<0.01

Digital Object Identifier (DOI)

10.1080/07853890.2024.2449225

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