Detection of codon 12 K-ras mutations in non-neoplastic mucosa from bronchial carina in patients with lung adenocarcinomas
2000
K-ras Mutations in Non-Cancerous Lung Tissue
Sample size: 19
publication
Author Information
Author(s): Urban T, Ricci S, Danel C, Antoine M, Kambouchner M, Godard V, Lacave R, Bernaudin J-F
Primary Institution: Hôpital Saint-Antoine
Hypothesis
The presence of cells containing K-ras mutation in non-neoplastic bronchial carina was investigated.
Conclusion
Activated K-ras by point mutation can be present in non-neoplastic bronchial carina mucosa even when no mutation is detected in tumor samples.
Supporting Evidence
- 42% of lung adenocarcinoma samples showed K-ras mutations.
- 47% of patients had no mutations detected in either tumor or bronchial carinae.
- 21% of patients had K-ras mutations in lung tumors but not in bronchial carinae.
- 21% of patients had K-ras mutations in both lung tumors and bronchial carinae.
- 11% of patients had K-ras mutations in bronchial carinae but not in lung tumors.
Takeaway
Scientists looked for a specific mutation in the lungs of patients with lung cancer and found it in some healthy tissue too.
Methodology
The study used restriction fragment length polymorphism enriched PCR method to detect mutations.
Digital Object Identifier (DOI)
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