A simple and rapid Hepatitis A Virus (HAV) titration assay based on antibiotic resistance of infected cells: evaluation of the HAV neutralization potency of human immune globulin preparations
2008
Rapid Hepatitis A Virus Titration Assay
publication
Evidence: high
Author Information
Author(s): Konduru Krishnamurthy, Virata-Theimer Maria Luisa, Yu Mei-ying W, Kaplan Gerardo G
Primary Institution: Food and Drug Administration
Hypothesis
Can a simple and rapid titration assay for Hepatitis A Virus (HAV) be developed using antibiotic resistance of infected cells?
Conclusion
The ARTA is a simple and rapid method to determine HAV titers without using HAV-specific probes.
Supporting Evidence
- The ARTA method allows for HAV titration in 8 days compared to 14 days required by traditional methods.
- The ARTA reduces labor, time, and cost of HAV titrations.
- The ARTA provides comparable results to the endpoint dilution ELISA method.
Takeaway
Scientists created a new way to quickly measure Hepatitis A virus levels using a special test that changes color, making it easier and faster than older methods.
Methodology
The study developed an antibiotic resistance titration assay (ARTA) using human hepatoma cells infected with a HAV construct containing a blasticidin resistance gene.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website