UTILLdb: A Database for Pea Genetics
Author Information
Author(s): Dalmais Marion, Schmidt Julien, Le Signor Christine, Moussy Francoise, Burstin Judith, Savois Vincent, Aubert Gregoire, Brunaud Veronique, de Oliveira Yannick, Guichard Cecile, Thompson Richard, Bendahmane Abdelhafid
Primary Institution: Unité de Recherche en Génomique Végétale, UMR INRA-CNRS
Hypothesis
To develop a high throughput forward and reverse genetics tool in pea, we have constructed a reference ethylmethane sulfonate mutant population and developed a database, UTILLdb.
Conclusion
The UTILLdb database provides a comprehensive resource for phenotypic and genetic information on mutant genes in pea, facilitating both forward and reverse genetics studies.
Supporting Evidence
- UTILLdb contains phenotypic and sequence information on mutant genes from a reference Pisum sativum EMS-mutant population.
- The study developed a high-quality P. sativum genetic mutant reference collection for forward and reverse genetics.
- The TILLING platform allows for the identification of mutations in targeted genes.
Takeaway
Researchers created a special database to help study pea plants that have been changed using a chemical to see how their genes work.
Methodology
The study involved creating a mutant population using EMS mutagenesis, followed by phenotyping and TILLING to identify mutations.
Limitations
The phenotyping tool does not cover all phenotypic alterations and was based on visual observation of only four mutant lines per M2 family.
Digital Object Identifier (DOI)
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