CpxR∼P Regulates RovA in Yersinia pseudotuberculosis
Author Information
Author(s): Liu Junfa, Obi Ikenna R., Thanikkal Edvin J., Kieselbach Thomas, Francis Matthew S.
Primary Institution: Umeå University, Umeå, Sweden
Hypothesis
CpxR phosphorylation negatively regulates the global regulator RovA in Yersinia pseudotuberculosis.
Conclusion
Cpx pathway activation restricts levels of the RovA global regulator, impacting virulence gene expression.
Supporting Evidence
- CpxR∼P binding to the rovA promoter inhibits transcription.
- In vivo accumulation of CpxR∼P reduces levels of RovA.
- CpxR∼P directly binds to the invasin promoter, affecting its expression.
Takeaway
When a certain protein gets a special tag, it helps the bacteria know when to stop making a key helper protein that helps them cause disease.
Methodology
The study characterized CpxR phosphorylation and its effects on RovA expression using various assays including EMSA and western blotting.
Limitations
The study does not explore all potential regulatory interactions affecting RovA expression.
Digital Object Identifier (DOI)
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