How Protein Phosphatase 6 Activates DNA-PK in Response to Radiation
Author Information
Author(s): Mi Jun, Dziegielewski Jaroslaw, Bolesta Elzbieta, Brautigan David L., Larner James M.
Primary Institution: University of Virginia Health System
Hypothesis
Does the association of PP6R1/PP6 with DNA-PK have a functional consequence in terms of kinase activation in response to ionizing radiation?
Conclusion
PP6R1 and PP6 are essential for the activation of DNA-PK in response to ionizing radiation, which is crucial for DNA repair.
Supporting Evidence
- DNA-PKcs associates with PP6R1, and this binding increases after ionizing radiation.
- Knockdown of PP6R1 or PP6 significantly decreased IR activation of DNA-PK.
- Cells lacking DNA-PK showed increased sensitivity to radiation-induced cell death.
Takeaway
This study shows that a protein called PP6 helps another protein, DNA-PK, work better when cells are damaged by radiation, which is important for fixing DNA.
Methodology
The study involved using glioblastoma cell lines, siRNA knockdown, immunoprecipitation, and various assays to analyze protein interactions and activities.
Limitations
The study primarily focuses on specific cell lines and may not fully represent all cellular contexts.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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