Host Transcript Accumulation during Lytic KSHV Infection Reveals Several Classes of Host Responses
2007
Host Gene Responses During KSHV Infection
Sample size: 10006
publication
10 minutes
Evidence: high
Author Information
Author(s): Chandriani Sanjay, Ganem Don
Primary Institution: University of California at San Francisco
Hypothesis
How does KSHV infection affect host gene expression?
Conclusion
KSHV infection leads to a significant reduction in host gene expression, with only a small percentage of transcripts escaping degradation.
Supporting Evidence
- Over 75% of host transcripts are substantially reduced during lytic infection.
- Approximately 20% of cellular mRNAs decline only slightly during infection.
- About 2% of examined cellular genes are strongly upregulated during lytic infection.
Takeaway
When KSHV infects cells, most of the genes stop working, but a few manage to keep going.
Methodology
Microarray analysis was used to assess host gene expression changes during KSHV lytic infection.
Potential Biases
Potential bias in transcript detection due to normalization methods.
Limitations
The study may not account for all potential host responses to KSHV infection.
Participant Demographics
TIME cells, a microvascular endothelial cell line.
Statistical Information
P-Value
1.0×10−3
Statistical Significance
p=1.0×10−3
Digital Object Identifier (DOI)
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