FMLP- and TNF-stimulated monoclonal Lym-1 antibody-dependent lysis of B lymphoblastoid tumour targets by neutrophils
1999
Neutrophils and Antibody-Dependent Cell Cytotoxicity
publication
Evidence: moderate
Author Information
Author(s): Ottonello L, Morone P, Mancini M, Amelotti M, Dapino P, Dallegri F
Primary Institution: University of Genova Medical School
Hypothesis
Can neutrophils induce cytolysis of B lymphoblastoid tumor targets when stimulated by specific antibodies and factors?
Conclusion
Neutrophils can effectively lyse B lymphoblastoid tumor cells when stimulated by the Lym-1 antibody in the presence of FMLP or TNF-α.
Supporting Evidence
- Neutrophils induced significant Cr51 release indicating cytolysis.
- The lytic process was inhibited by specific antibodies targeting Fcγ receptors.
- FMLP and TNF-α were found to synergize in promoting neutrophil cytolytic potential.
Takeaway
This study shows that certain immune cells can kill cancer cells when helped by specific signals and antibodies.
Methodology
Human neutrophils were incubated with Cr51-labelled B lymphoblastoid Raji cells and stimulated with Lym-1 antibody and either FMLP or TNF-α to assess cytolysis.
Digital Object Identifier (DOI)
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