How miR-125b Regulates the p53 Network
Author Information
Author(s): Le Minh T. N., Shyh-Chang Ng, Khaw Swea Ling, Chin Lingzi, Teh Cathleen, Tay Junliang, O'Day Elizabeth, Korzh Vladimir, Yang Henry, Lal Ashish, Lieberman Judy, Lodish Harvey F., McManus Michael T.
Primary Institution: Genome Institute of Singapore
Hypothesis
What genes in the p53 network might be regulated by miR-125b?
Conclusion
miR-125b regulates the p53 network by directly repressing 20 novel targets, affecting both apoptosis and cell-cycle regulation.
Supporting Evidence
- miR-125b directly represses 20 novel targets in the p53 network.
- The regulation of the p53 pathway by miR-125b is conserved at the network level.
- miR-125b modulates both apoptosis and cell-cycle regulators.
Takeaway
This study shows that a tiny molecule called miR-125b helps control important genes that decide if cells should grow or die, which is important for keeping our bodies healthy.
Methodology
The study used gain- and loss-of-function screens, luciferase assays, and miRNA pull-down assays to identify and validate miR-125b targets in humans, mice, and zebrafish.
Limitations
The study may not identify rapidly degraded mRNA targets and relies on specific experimental conditions.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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