An 11-bp Insertion in Zea mays fatb Reduces the Palmitic Acid Content of Fatty Acids in Maize Grain
2011

Genetic Analysis of Fatty Acids in Maize Grain

Sample size: 877 publication 10 minutes Evidence: high

Author Information

Author(s): Li Lin, Li Hui, Li Qing, Yang Xiaohong, Zheng Debo, Warburton Marilyn, Chai Yuchao, Zhang Pan, Guo Yuqiu, Yan Jianbing, Li Jiansheng

Primary Institution: National Maize Improvement Center of China, China Agricultural University, Beijing, China

Hypothesis

An 11-bp insertion in the fatb gene reduces palmitic acid content in maize grain.

Conclusion

The study identifies an 11-bp insertion in the fatb gene that decreases palmitic acid content in maize, improving the ratio of saturated to unsaturated fatty acids without affecting total oil content.

Supporting Evidence

  • An 11-bp insertion in the fatb gene was identified as the causal polymorphism affecting palmitic acid content.
  • The study measured the genetic effect of the functional site in 15 different genetic backgrounds.
  • A PCR-based marker for QTL-Pal9 was developed for marker-assisted selection of healthier maize lines.
  • The 11-bp InDel was significantly associated with variation in palmitic acid content across multiple environments.

Takeaway

Scientists found a tiny change in a gene that helps make healthier corn oil by lowering a type of fat called palmitic acid.

Methodology

The study combined linkage and association analyses to fine map QTL-Pal9 and identify the candidate gene Zmfatb.

Limitations

The study does not address the potential effects of other fatb iso-enzymes on palmitic acid content.

Statistical Information

P-Value

5.0E−14

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0024699

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