Effects of Substituted Trans-Stilbenes on AP-1 Activation
Author Information
Author(s): Deck Lorraine M, Hunsaker Lucy A, Gonzales Amanda M, Orlando Robert A, Vander Jagt David L
Primary Institution: University of New Mexico
Hypothesis
Can substituted trans-stilbenes inhibit or enhance the TPA-induced activation of activator protein-1 (AP-1)?
Conclusion
Trans-stilbenes can inhibit or enhance the effects of TPA without relying on their anti-oxidant properties.
Supporting Evidence
- Several trans-stilbenes were identified that inhibit TPA-induced activation of AP-1, with IC50 values as low as 0.5 μM.
- Some trans-stilbenes enhanced the effects of TPA 2 to 3-fold.
- Many identified trans-stilbenes are devoid of anti-oxidant properties.
Takeaway
Some plant compounds can either help or hurt a specific protein's activity in cells, and this doesn't depend on whether they are antioxidants.
Methodology
A library of substituted trans-stilbenes was screened using the Panomics AP-1 Reporter 293 Stable Cell Line to assess their effects on TPA-induced AP-1 activation.
Limitations
The study did not identify the specific targets of the trans-stilbenes affecting AP-1 activity.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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