Understanding Drug Resistance in Rhabdomyosarcoma
Author Information
Author(s): Cocker H A, Pinkerton C R, Kelland L R
Primary Institution: The Institute of Cancer Research
Hypothesis
The role of multidrug resistance (MDR) and p53 functional status in the treatment of paediatric rhabdomyosarcoma is unclear.
Conclusion
Some rhabdomyosarcoma cell lines exhibit low levels of multidrug resistance, which may affect their sensitivity to certain drugs like vincristine.
Supporting Evidence
- None of the cell lines had detectable P-glycoprotein or multidrug resistance-related protein.
- RT-PCR studies indicated the presence of mdr-1, mrp-1, and lrp mRNA in several lines.
- The function of p53 is compromised in six of the lines.
- Vincristine sensitivity could be modulated significantly using PSC833 and VX710.
Takeaway
Researchers studied cancer cells from kids to see how they resist treatment, finding that some cells can be more resistant to a common drug.
Methodology
The study characterized seven human rhabdomyosarcoma cell lines for MDR and p53 phenotype using Western blotting, immunohistochemistry, and RT-PCR.
Limitations
The levels of MDR proteins were very low and difficult to detect.
Participant Demographics
Human paediatric rhabdomyosarcoma cell lines.
Digital Object Identifier (DOI)
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