Mutagenic Activity of Brazilian Propolis in Topical Formulations
Author Information
Author(s): Senedese Juliana Marques, Rodrigues Aline Rafaela, Furtado Michelle Andrade, Faustino Viviane Dias, Berretta Andresa A., Marchetti Juliana M., Tavares Denise Crispim
Primary Institution: Universidade de Franca
Hypothesis
Does the topical application of Brazilian green propolis extract induce mutagenic effects in mammalian cells?
Conclusion
Topical formulations containing different concentrations of green propolis showed no mutagenic effect, but the 3.6% propolis gel was cytotoxic in vitro.
Supporting Evidence
- Propolis has various biological activities including antibacterial and antioxidant properties.
- The study evaluated mutagenic potential through chromosomal aberrations and micronuclei analysis.
- 3-h pulse and continuous treatments were performed in vitro on CHO cells.
- In vivo tests showed no increase in micronuclei frequency in treated rats.
- 3.6% propolis gel was found to be cytotoxic in vitro.
- Topical formulations did not show mutagenic effects under the tested conditions.
Takeaway
This study tested creams with propolis to see if they could harm cells. They found that while the creams didn't cause damage, the strongest one could be harmful to cells in a lab.
Methodology
The study involved in vitro analysis of chromosomal aberrations in CHO cells and in vivo micronucleus tests in Wistar rats using topical formulations with varying concentrations of propolis.
Limitations
The study did not assess long-term effects or systemic absorption of propolis when applied topically.
Participant Demographics
30 male Wistar rats were used for the in vivo tests.
Statistical Information
Statistical Significance
p>0.05
Digital Object Identifier (DOI)
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