Caryophyllene Oxide from Annona macroprophyllata Shows Antitumor Activity
Author Information
Author(s): Jesica Ramírez-Santos, Fernando Calzada, Normand García-Hernández, Elizabeth Barbosa, Claudia Velázquez, Miguel Valdes, Marta Menegazzi
Primary Institution: Unidad de Investigación Médica en Farmacología, UMAE Hospital de Especialidades, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico
Hypothesis
The study aimed to investigate the anticancer activity of caryophyllene oxide isolated from Annona macroprophyllata using in vivo, in vitro, and in silico approaches.
Conclusion
Caryophyllene oxide exhibited significant antilymphoma and cytotoxic activities, comparable to methotrexate, and is a potential anticancer agent.
Supporting Evidence
- Caryophyllene oxide showed better cytotoxic activity compared to methotrexate.
- Flow cytometry indicated that caryophyllene oxide induced late apoptosis and necrosis in U-937 cells.
- Acute oral toxicity results classified caryophyllene oxide as low risk with an LD50 > 1000 mg/kg.
Takeaway
Caryophyllene oxide, a compound from a plant, can help fight a type of cancer called lymphoma and is safe for use.
Methodology
The study used gas chromatography-mass spectroscopy for identification, evaluated cytotoxic activity with the WST method, and assessed antilymphoma activity in mice.
Limitations
The study's in vivo model may not fully replicate human tumor environments, and the acute toxicity was assessed with a single dose.
Participant Demographics
Male and female Balb/c mice were used for the in vivo studies.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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