CompMoby: Comparative MobyDick for detection of cis-regulatory motifs
2008

CompMoby: A Tool for Finding Gene Regulation Motifs

Sample size: 41 publication Evidence: high

Author Information

Author(s): Christina Chaivorapol, Collin Melton, Grace Wei, Ru-Fang Yeh, Miguel Ramalho-Santos, Robert Blelloch, Hao Li

Primary Institution: University of California, San Francisco

Hypothesis

Can computational methods effectively identify cis-regulatory motifs in metazoans?

Conclusion

CompMoby is a powerful software tool for discovering both conserved and nonconserved cis-regulatory sequences involved in gene regulation.

Supporting Evidence

  • CompMoby successfully identified cis-regulatory target sites of known and novel trans-acting factors.
  • The software allows detection of motifs in alignable, conserved, and species-specific categories.
  • Experimental validation showed significant luciferase reporter activity for predicted motifs.

Takeaway

CompMoby helps scientists find important DNA sequences that control how genes work, making it easier to study gene regulation.

Methodology

The CompMoby software uses a computational algorithm to identify cis-regulatory binding sites in gene sequences without prior knowledge of the trans-acting factors.

Limitations

The algorithm may not capture all regulatory elements due to the complexity of gene regulation in metazoans.

Statistical Information

P-Value

p<0.001

Statistical Significance

p<0.001

Digital Object Identifier (DOI)

10.1186/1471-2105-9-455

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