Genome sequence of adherent-invasive Escherichia coli and comparative genomic analysis with other E. coli pathotypes
2010
Genome sequence of adherent-invasive Escherichia coli and comparative genomic analysis
publication
Evidence: high
Author Information
Author(s): Nash John HE, Villegas Andre, Kropinski Andrew M, Aguilar-Valenzuela Renan, Konczy Paulina, Mascarenhas Mariola, Ziebell Kim, Torres Alfredo G, Karmali Mohamed A, Coombes Brian K
Primary Institution: Public Health Agency of Canada
Hypothesis
What are the genetic determinants accounting for the AIEC phenotype?
Conclusion
The genome sequence data of AIEC will help identify genetic factors involved in invasion and growth within host cells.
Supporting Evidence
- The genome sequence confirmed a link between AIEC and other pathogenic E. coli.
- 41 genomic islands and unique genes were identified in the AIEC pathotype.
- AIEC strains are associated with strong inflammatory responses in the gut.
- Genomic islands may play a role in the expression of the AIEC phenotype.
Takeaway
Scientists sequenced the DNA of a type of E. coli that can invade the gut and cause inflammation, which may help us understand Crohn's disease better.
Methodology
The genome of E. coli NRG857c was sequenced using pyrosequencing and analyzed for unique genetic features.
Digital Object Identifier (DOI)
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