IFNG +874T/A Polymorphism and Leishmaniasis
Author Information
Author(s): Matos Guilherme Inocêncio, Covas Claudia de J Fernandes, Bittar Rita de Cássia, Gomes-Silva Adriano, Marques Fabiana, Maniero Viviane C, Amato Valdir S, Oliveira-Neto Manoel P, Mattos Marise da Silva, Pirmez Claude, Sampaio Elizabeth P, Moraes Milton O, Da-Cruz Alda Maria
Primary Institution: Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil
Hypothesis
Does the IFNG +874T/A SNP influence susceptibility to American tegumentary leishmaniasis and IFN-γ production?
Conclusion
The IFNG +874T/A polymorphism is not associated with susceptibility to leishmaniasis but may influence IFN-γ production in cutaneous leishmaniasis patients.
Supporting Evidence
- The study included 136 leishmaniasis patients and 609 healthy controls.
- Genotyping was performed using Amplification Refractory Mutational System (ARMS-PCR).
- IFN-γ production was assessed by ELISA in PBMC cultures.
- No association was found between the SNP and susceptibility to leishmaniasis.
- Lower IFN-γ levels were observed in CL patients with the AA genotype.
- High and low IFN-γ producers were identified among ML patients.
- Statistical analysis showed significant differences in IFN-γ production between CL and ML patients.
- Results suggest that other regulatory genes may also influence IFN-γ production.
Takeaway
This study looked at a gene that might affect how our body fights a disease called leishmaniasis. They found that while the gene doesn't make you more likely to get sick, it can change how much of a certain helper protein your body makes.
Methodology
The study involved genotyping the IFNG +874T/A SNP in 136 leishmaniasis patients and 609 healthy controls, assessing IFN-γ production in vitro.
Limitations
The study may not account for other genetic factors influencing leishmaniasis susceptibility.
Participant Demographics
78 cutaneous leishmaniasis patients (50 males, 28 females) and 58 mucosal leishmaniasis patients (39 males, 19 females) from Rio de Janeiro, Brazil.
Statistical Information
P-Value
p = 0.59 for genotypes and p = 0.4735 for alleles
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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