Microsatellite Markers for Sorghum Downy Mildew
Author Information
Author(s): Perumal Ramasamy, Nimmakayala Padmavathi, Erattaimuthu Saradha R, No Eun-Gyu, Reddy Umesh K, Prom Louis K, Odvody Gary N, Luster Douglas G, Magill Clint W
Primary Institution: Texas A&M University
Hypothesis
Can simple sequence repeat markers be developed to differentiate between isolates of Peronosclerospora sorghi and related species?
Conclusion
The study successfully developed microsatellite markers that can distinguish between different isolates of downy mildew pathogens.
Supporting Evidence
- 36 microsatellite loci were identified from the genomic DNA of Peronosclerospora sorghi.
- Successful cross-amplification was achieved for 12 primer pairs across related downy mildew species.
- A total of 260 alleles were obtained using 54 microsatellite primer combinations.
Takeaway
Scientists created special DNA markers to tell different types of plant diseases apart, which can help farmers protect their crops.
Methodology
The study used a biotin capture method to discover and clone microsatellite markers from the genomic DNA of Peronosclerospora sorghi.
Limitations
The study may not cover all possible isolates and the results are based on a limited number of samples.
Digital Object Identifier (DOI)
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