Developing Genetic Markers for Yam Disease Resistance
Author Information
Author(s): Narina Satya S, Buyyarapu Ramesh, Kottapalli Kameswara Rao, Sartie Alieu M, Ali Mohamed I, Robert Asiedu, Hodeba Mignouna JD, Sayre Brian L, Scheffler Brian E
Primary Institution: USDA-ARS, Stoneville, MS, USA
Hypothesis
The study aims to generate expressed sequence tags (ESTs) to identify molecular markers associated with anthracnose resistance in yam.
Conclusion
The research successfully developed a comprehensive annotated transcriptome dataset for yam, aiding in the identification of unique transcripts related to disease resistance.
Supporting Evidence
- A total of 44,757 EST sequences were generated from the cDNA libraries.
- More than 56% of ESTs were annotated using bioinformatics tools.
- 1705 SSR markers were designed from the identified unique sequences.
Takeaway
Scientists studied different types of yam plants to find out which ones can resist a harmful fungus, and they created a big list of genes that can help improve yam crops.
Methodology
Total RNA was isolated from yam leaves, and cDNA libraries were sequenced using Roche 454 technology to generate ESTs.
Limitations
Approximately 50% of ESTs could not be annotated and remained unknown.
Digital Object Identifier (DOI)
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