Rapid Detection of Isocarbophos in Fruits and Vegetables
Author Information
Author(s): Chen Zijian, Huang Wei-Xuan, Wang Hongwu, Zhang Meiling, Chen Kai, Deng Hao, Noguer Thierry
Primary Institution: Key Laboratory of Tropical Fruit and Vegetable Cold-Chain of Hainan Province, Institute of Agro-Products of Processing and Design, Hainan Academy of Agricultural Sciences, Haikou, China
Hypothesis
Can a multicolor immunoassay be developed for the rapid detection of isocarbophos in vegetable and fruit samples?
Conclusion
The developed multicolor immunoassay allows for rapid and accurate detection of isocarbophos without the need for bulky instruments.
Supporting Evidence
- The immunoassay achieved a color conversion from orange to blue in response to isocarbophos concentration.
- Smartphone RGB analysis provided a portable solution for on-site detection.
- The method showed good accuracy and practicability in recovery tests.
- Calibration curves indicated a limit of detection of 4.6 ng/mL for the immunoassay.
- Recovery rates for the multicolor immunoassay ranged from 81.8% to 112.4%.
Takeaway
This study created a simple test that changes color to show if a harmful pesticide is in fruits and vegetables, making it easy to check for safety.
Methodology
The study developed a multicolor immunoassay using a competitive immunoassay approach with color changes measured by absorbance and smartphone RGB analysis.
Limitations
The RGB analysis requires manual selection of microplate locations and may have accuracy issues due to field-of-view mismatches.
Digital Object Identifier (DOI)
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