Hypoxia significantly reduces aminolaevulinic acid-induced protoporphyrin IX synthesis in EMT6 cells
1999
Effects of Hypoxia on Protoporphyrin IX Synthesis
publication
Author Information
Author(s): Georgakoudi I, Keng P C, Foster T H
Primary Institution: University of Rochester School of Medicine and Dentistry
Hypothesis
How does hypoxia affect aminolaevulinic acid-induced protoporphyrin IX synthesis in different cell populations?
Conclusion
Hypoxia significantly reduces protoporphyrin IX synthesis, with the effect varying by cell density and proliferation rate.
Supporting Evidence
- The amount of protoporphyrin IX produced was higher after normoxic incubation.
- A 42-fold decrease in protoporphyrin IX fluorescence was observed for high-density unfed-plateau cells.
- Low-density exponential cells were least affected by hypoxia.
Takeaway
When cells are in low oxygen, they make less of a special compound called protoporphyrin IX, and how much less depends on how crowded the cells are.
Methodology
The study measured protoporphyrin IX content in EMT6 cell cultures under hypoxic and normoxic conditions after aminolaevulinic acid incubation.
Digital Object Identifier (DOI)
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