Genomic Sequence and Activity of KS10, a Transposable Phage of Burkholderia cepacia
Author Information
Author(s): Goudie Amanda D, Lynch Karlene H, Seed Kimberley D, Stothard Paul, Shrivastava Savita, Wishart David S, Dennis Jonathan J
Primary Institution: University of Alberta
Hypothesis
Does the prophage KS10 encode any virulence determinants?
Conclusion
KS10 is a novel phage with a unique genomic organization and no identified potential virulence factors.
Supporting Evidence
- KS10 is a 37,635 base pairs transposable phage of Burkholderia cenocepacia.
- KS10 was found to be a prophage in three different strains of B. cenocepacia.
- A survey of 23 strains and 20 clinical isolates revealed that KS10 can form plaques on certain BCC strains.
Takeaway
Researchers studied a virus that infects bacteria called KS10, and found it doesn't have genes that make bacteria harmful.
Methodology
The genome of KS10 was sequenced using a shotgun cloning and sequencing approach, and its host range was tested using plaque assays.
Limitations
The study could not determine the exact integration sites of KS10 in all known BCC hosts due to mispriming in PCR experiments.
Digital Object Identifier (DOI)
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