Improved Proteome Coverage with iTRAQ and OFFGEL Fractionation
Author Information
Author(s): Ernoult Emilie, Gamelin Erick, Guette Catherine
Primary Institution: Centre INSERM Régional de Recherche sur le Cancer U892, Centre Régional de Lutte Contre le Cancer Paul Papin, Angers, France
Hypothesis
Does the combination of iTRAQ labelling and OFFGEL fractionation improve proteome coverage in complex samples?
Conclusion
The combined use of iTRAQ labelling and OFFGEL fractionation significantly increases proteome coverage and aids in identifying low-concentration proteins.
Supporting Evidence
- iTRAQ labelling allowed the identification of 2.7 times more proteins compared to free-labelled samples.
- The OFFGEL fractionation method increased the number of identified proteins significantly.
- The study identified a total of 947 proteins with at least 2 peptides.
Takeaway
This study shows that using special labels and a new way to separate proteins helps scientists find more proteins in complex samples.
Methodology
The study used iTRAQ labelling and OFFGEL fractionation to analyze proteins from the human neuroblastoma SH-SY5Y cell line.
Limitations
The study may have limitations related to the complexity of the samples and the potential for bias in protein identification.
Participant Demographics
Human neuroblastoma SH-SY5Y cell line.
Digital Object Identifier (DOI)
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