Gene Expression Profiling in Whole Blood Samples
Author Information
Author(s): Winn Mary E, Shaw Marian, April Craig, Klotzle Brandy, Fan Jian-Bing, Murray Sarah S, Schork Nicholas J
Primary Institution: University of California at San Diego
Hypothesis
The Illumina whole genome DASL assay can detect gene expression levels using partially degraded RNA samples without the need for globin reduction.
Conclusion
The detection sensitivity of the whole genome DASL assay is higher than the IVT-based direct hybridization assay, with or without globin reduction.
Supporting Evidence
- Gene expression detection is significantly increased with the use of whole genome DASL compared to the standard IVT-based direct hybridization.
- Globin reduction further increases the detection sensitivity and reliability of both whole genome DASL and IVT-based direct hybridization.
- 8677 probes were detected across all samples by the five target preparation methods.
Takeaway
This study shows that a new method can better detect genes in blood samples without needing to remove certain types of RNA, making it easier to find important health markers.
Methodology
Peripheral whole blood samples were collected from eight human donors, RNA was isolated, and gene expression was analyzed using the Illumina Human-Ref8 v3.0 Beadchip.
Limitations
The study was not designed to address the ability of the WG-DASL assay to distinguish between disease populations.
Participant Demographics
Eight human donors were used in the study.
Statistical Information
P-Value
2.13 × 10-9
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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