Transcutaneous CO2 Application and the Bohr Effect
Author Information
Author(s): Sakai Yoshitada, Miwa Masahiko, Oe Keisuke, Ueha Takeshi, Koh Akihiro, Niikura Takahiro, Iwakura Takashi, Lee Sang Yang, Tanaka Masaya, Kurosaka Masahiro
Primary Institution: Kobe University Graduate School of Medicine
Hypothesis
Does transcutaneous application of CO2 cause the Bohr effect in the human body?
Conclusion
The study provides evidence that transcutaneous CO2 application facilitates oxygen dissociation from hemoglobin in the human body.
Supporting Evidence
- The rat skin experiment showed that CO2 hydrogel enhanced CO2 gas permeation through the rat skin.
- The intracellular pH of the triceps surae muscle decreased significantly 10 min. after transcutaneous application of CO2.
- Oxy-Hb concentration significantly decreased while deoxy-Hb concentration significantly increased after transcutaneous CO2 application.
Takeaway
This study shows that applying CO2 through the skin can help oxygen get released from blood cells, which is good for treating certain health problems.
Methodology
The study used a novel system for transcutaneous CO2 application and measured pH changes and hemoglobin concentrations in human subjects.
Potential Biases
Potential conflicts of interest due to funding from NeoChemir Inc.
Limitations
The study's findings may be influenced by the presence of myoglobin in NIRS measurements.
Participant Demographics
Five healthy male volunteers aged 23-38 and seven healthy male volunteers aged 27-40.
Statistical Information
P-Value
0.0003
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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