SRp20 Re-localization during Poliovirus Infection
Author Information
Author(s): Fitzgerald Kerry D., Semler Bert L.
Primary Institution: Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, California, United States of America
Hypothesis
How does the interaction between cellular proteins PCBP2 and SRp20 affect poliovirus translation initiation?
Conclusion
SRp20 re-localizes from the nucleus to the cytoplasm during poliovirus infection and interacts with PCBP2 to facilitate viral translation.
Supporting Evidence
- SRp20 re-localizes to the cytoplasm of infected cells starting about 2 hours post-infection.
- SRp20 and PCBP2 co-sediment in translation initiation complex-containing fractions.
- Expression of a mutated version of SRp20 results in a significant decrease in poliovirus yield.
Takeaway
When a virus infects a cell, a protein called SRp20 moves from the cell's nucleus to the cytoplasm to help the virus make more copies of itself.
Methodology
The study used imaging techniques, fractionation assays, and RNA binding experiments to analyze the role of SRp20 and PCBP2 in poliovirus translation.
Digital Object Identifier (DOI)
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